View of Acetaminophen, the missing anion gap in the patient with metabolic acidosis The Southwest journal of medicine Tylenol's Impact on Glutathione and Liver Damage Can Tylenol (acetaminophen) deplete glutathione levels in the body? Let's talk Tylenol Paracetamol (acetaminophen) There are many side effects that most people don't realize. OTC does not mean it is side effect free, especially with chronic use. Even just 4 days of Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.